Page 63 - Kutnar, Andreja, et al., eds., 2015. Proceedings of the 1st COST Action FP1307 International Conference - Life Cycle Assessment, EPDs, and modified wood. University of Primorska Press, Koper.
P. 63
 lower
 phenolic
 and
 flavonoid
 contents
 on
 crude
 extract
 could
 be
 explained
 by
 the
 presence
 of
 
impurities
  such
  as
  organic
  acids,
  which
  can
  interfere
  with
  the
  determination
  of
  the
  chemical
 
composition
  (Chirinos
  et
  al.
  2007).
  The
  butanolic
  fraction
  exhibited
  the
  most
  effective
  DPPH
 
scavenging
 capacity,
 followed
 by
 ethyl
 acetate
 fraction
 and
 crude
 extract.
 According
 to
 Dudonné
 
et
  al.
  2009,
  the
  presence
  of
  phenolic
  compounds
  in
  the
  plant
  contributes
  strongly
  to
  their
 
antioxidant
  ability.
  Following
  the
  TGA
  analysis,
  the
  first
  step
  of
  decomposition
  in
  all
  extracts
 
corresponded
 to
 dehydration
 (<180°C).
 In
 crude
 extractives,
 the
 degradation
 process
 occurred
 at
 
250°C
 and
 at
 400°C,
 while
 the
 fractions
 showed
 a
 main
 step
 approximately
 at
 300°C
 (Fig.
 1).
 This
 
difference
 may
 be
 due
 to
 decomposition
 of
 crude
 extractives
 in
 different
 fractions
 of
 compounds
 
(Ross
 et
 al.
 2009).
 The
 results
 of
 decay
 test
 are
 ongoing,
 but
 as
 a
 preliminary
 report
 the
 antifungal
 
activity
  found
  in
  the
  impregnated
  samples
  is
  related
  to
  the
  presence
  of
  phenolic
  compounds
  in
 
the
 O.
 acutifolia
 leaves
 extracts.
 

 


 

Figure
 1:
  Thermogravimetric
 analysis
 of
 O.
 acutifolia
 leaves
 extracts.
 

 

References
 

 
Chirinos
 R.,
 Rogez
 H.,
 Campos
 D.,
 Pedreschi
 R.,
 Larondelle
 Y.
 2007.
 Optimization
 of
 extraction
 conditions
 of
 

antioxidant
  phenolic
  compounds
  from
  mashua
  (Tropaeolum
  tuberosum
  Ruíz
  &Pavón)
  tubers.
 
Separation
 and
 Purification
 Technology,
 55:
 217–225.
 
Dudonné
  S.,
  Vitrac
  X.,
  Coutière
  P.,
  Woillez
  M.,
  Mérillon
  J-­‐M.
  2009.
  Comparative
  Study
  of
  Antioxidant
 
Properties
 and
 total
 phenolic
 content
 of
 30
 plant
 extracts
 of
 industrial
 interest
 using
 DPPH,
 ABTS,
 FRAP,
 
SOD,
 and
 ORAC
 assays.
 Journal
 of
 A
 gricultural
 and
 Food
 Chemistry,
 57:
 1768–1774.
 
Egua
  M.
  O.,
  Etuk
  E.
  U.,
  Bello
  S.
  O.,
  Hassan
  S.
  W.
  2014.
  Antidiabetic
  potential
  of
  liquid-­‐liquid
  partition
 
fractions
 of
 ethanolic
 seed
 extract
 of
 Corchorus
 olitorious.
 Journal
 of
 Pharmacognosy
 and
 Phytotherapy,
 
6,
 1:
 4-­‐9.
 
Ross
  A.
  B.,
  Anastasakis
  K.,
  Kubacki
  M.,
  Jones
  J.
  M.
  2009.
  Investigation
  of
  the
  pyrolysis
  behavior
  of
  brown
 
algae
  before
  and
  after
  pre-­‐treatment
  using
  PY-­‐GC-­‐MS
  and
  TGA.
  Journal
  of
  Analytical
  and
  Applied
 
Pyrolysis,
 85:
 3-­‐10.
 


 

Acknowledgments
 

The
  authors
  are
  grateful
  for
  the
  scholarship
  of
  Brazilian
  National
  Counsel
  of
  Technological
  and
 

Scientific
  Development
  (CNPq)
  and
  for
  the
  financial
  support
  of
  the
  Basque
  Government
  (project
 

IT672-­‐13).
 
 


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